Notably, the fluorescent in situ hybridization (FISH) assays detected six and four Myc amplicons in the majority of Ctdnep1-cKO tumor cells (17/20) and Ctdnep1-cKO NPCs (14/20) at DIC 70, respectively, compared with the normal two Myc gene copies in control NPCs (Fig. 5l). This evidence concerns the gene CTDNEP1 and neoplasm.