NAT10 and hepatocellular carcinoma: Western blot and qPCR in normal hepatocytes LO2 and four HCC cell lines (Huh-7, Hep3B, MHCC97h, and SK-HEP-1) also showed that protein and RNA levels of NAT10 were upregulated in Huh-7 and hep3B HCC cell lines compared with LO2 cell line (Fig. 1D, E).