OGT and hepatocellular carcinoma: To confirm that O-GlcNAcylation of YTHDF2 is mediated by OGT, co-immunoprecipitation (Co-IP) and confocal assays were performed to demonstrate the interaction between OGT and YTHDF2 in HEK293 and HBV-infected hepatoma cells (Fig. 2a–c and Supplementary Fig. 2a).