Moreover, sWGA pull-down assays in hepatoma cells and HEK293 cells indicated that HBV infection or TMG treatment significantly enhanced total O-GlcNAcylation and YTHDF2 O-GlcNAcylation (Supplementary Fig. 1b–g and Fig. 1h, i), whereas the OGT inhibitor OSMI-1 downregulated both processes (Supplementary Fig. 1h, i and Fig. 1j, k). Here, OGT is linked to hepatocellular carcinoma.