To verify if the iron manipulation has an impact on iron-related proteins and on the intracellular iron content, the RD (among the Embryonal Rhabdomyosarcoma cell lines previously used) and RH30 (among the Alveolar Rhabdomyosarcoma cell lines previously used) have been treated with Ferric Ammonium Citrate (FAC, 100–500 μM for RD and 50–100 μM for RH30) or Deferiprone (DFP, 100–200 μM) for 16 h and iron content (in the form of labile iron pool, LIP), TfR1 and H-ferritin protein content were analyzed, as the main proteins involved in iron up-take and storage, respectively. This evidence concerns the gene FTH1 and rhabdomyosarcoma.