As shown in Fig 4A and 4B, HCK1T/16epi with expression of both MYC/PIK3CAE545K and MEK1DD resulted rapid tumor formation regardless of the early-passage or the late-passage; tumor volumes reached more than 600 mm3 in all mice (100%; 4 of 4 for each passage) within 4 weeks for early-passage and 3 weeks for late-passage cells. The gene discussed is MYC; the disease is neoplasm.