KTN1 and glioblastoma: To investigate the biological functions of KTN1-AS1 in GBM cells, first, KTN1-AS1 was upregulated in A172 cells by transfecting KTN1-AS1 overexpression plasmids and silenced in U251 cells by transfecting KTN1-AS1 siRNAs (Figures 2(a) and 2(b)).