In a real-world experiment, the RT-qPCR results were consistent with the bioinformatic analysis, confirming that ADAMTSL4, DOCK6, FAM111B, and SEMA6B were expressed at higher levels in thyroid cancer cells (p < 0.05), while MRPS10 and PSMB7 were expressed at lower levels (p < 0.05) (Figure 11C) (Supplementary Table S4). The gene discussed is SEMA6B; the disease is thyroid cancer.