A bispecific and split CAR-T (BissCAR) was produced against CD13, and TIM3 antigens of AML cells, in which CD13-Nb was linked to CD3ζ signaling and anti-TIM3-scFv was linked to two costimulatory domains, CD28 and 4-1BB. The gene discussed is HAVCR2; the disease is acute myeloid leukemia.