In A clinical study of 22 patients with renal cell carcinoma before and 3 months after cryoablation and blood samples of some patients, it was found that CD8+T, CD4+T, granzyme A(GZMA), CD11c transcription levels were significantly increased, and CD8/FOXP3 was increased after cryoablation. Here, GZMA is linked to renal cell carcinoma.