Another to investigate the impact of neoadjuvant immunotherapy on the tumor microenvironment at multiple levels, including genome, transcriptome, PD-1/PD-L1 protein transcription and expression, T cell TCR immunome library, and T cell subpopulation, aiming to provide comprehensive exploratory research evidence on immune mechanisms of neoadjuvant anti-PD-1/L1 therapy in lung cancer. This evidence concerns the gene CD274 and neoplasm.