Clearly, the validation dataset confirmed only ALAS2, SELENBP1, CD3G, MAP1LC3A, CD69, DCAF12, IGF2R, PSMC4, and SLC4A1 as significantly differently expressed hub genes between children with ASD and TD and not the genes AHSP, AP2A1, BCL2L1, EPB42, GMPR, and LILRB. This evidence concerns the gene PSMC4 and thanatophoric dysplasia.