Using the CytoHubba function, there were a total of 15 hub genes (Figure 6A), including AHSP, ALAS2, SELENBP1, AP2A1, BCL2L1, CD3G, MAP1LC3A, CD69, DCAF12, EPB42, GMPR, IGF2R, LILRB1, PSMC4, and SLC4A1, and the expression levels of the hub genes were determined in the training (Figure 6B) and validation (Figure 6C) datasets in children with ASD and TD. Here, MAP1LC3A is linked to thanatophoric dysplasia.