Figure 3 also shows the protein’s position in the ordered list, the corresponding expression level, and the ratio from the comparison. Furthermore, Figure 3C displays a heatmap of the related proteins enriched in the PD pathway. SIRT4 overexpression increases the expression of related proteins in the PD pathway, which play a protective role in PD. In summary, a GSEA of the proteomics results from the in vitro model supports the notion that SIRT4 significantly participates in PD by exerting protective effects. The gene discussed is SIRT4; the disease is Parkinson disease.