In addition, to evaluate the therapeutic effect, hematoxylin and eosin (H&E) staining and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining were performed on the primary tumor to observe the necrosis and apoptosis, and the proliferation of the metastatic tumor was assessed by PCNA staining (Fig. S6e). The gene discussed is PCNA; the disease is neoplasm.