TP53 and central nervous system cancer: To further analyze whether P53 signaling was downregulated by the FGL2-blocking scFv, Gene Set Enrichment Analysis (GSEA) was performed, revealing that the expression of genes associated with P53 signaling was lower in T-αFGL2 cells than in T-Ctr cells in the context of coculture with glioma cells (Supplementary Fig. 8e, normalized enrichment score [NES] = −2.0, P = 0.009).