PRKN and heart failure: However, cardiac senescence determined by senescence-associated β-galactosidase and mitochondrial function evaluated by ATP5A expression were significantly improved after a single administration of AAV-Parkin-T2A-GFP compared with AAV-GFP administration (Fig. S4, J–O), suggesting the benefit of the Parkin gene transfer in RhoA cKO mice even after mild heart failure begins.