For infection of cells with SARS-CoV-2 pseudovirus in cis inhibition assays, WT HEK293T, HEK293T-ACE2, and HEK293T-ACE2-TMPRSS2 cells were transfected with cDNA for myc-DDK-tagged LRRC15 transcript 1 or a control plasmid (lentiGuide-Puro; Addgene #52963). Here, ACE2 is linked to infection.