Furthermore, ectopic PI4K2A expression in epithelial H441 cells enhanced cell proliferation and tumor growth and metastasis in mice (Supplemental Figure 3, G–J), and PI4K2A depletion in mesenchymal lung cancer cells increased apoptosis in a monolayer culture, decreased colony formation in soft agar, and reduced migration and invasion in Boyden chambers (Figure 3, M–O, and Supplemental Figure 3, K–P). This evidence concerns the gene PI4K2A and lung cancer.