Finally, to determine whether the level of SAMD9 is directly associated with ESCC recurrence, when the mean tumor volume reached ∼45 mm3, mice inoculated with ECA109‐vector and ECA109‐SAMD9 or TE1‐scramble and TE1‐shRNA of SAMD9 cells began receiving CDDP (cis‐diaminedichloroplatinum) treatment. The gene discussed is SAMD9; the disease is esophageal squamous cell carcinoma.