To confirm this conjecture, we first performed qRT‐PCR for the detection of miR‐542‐3p and SFRP1 expression in NHAs and U87 and U251 glioma cells, and a high expression of miR‐542‐3p and a low expression of SFRP1 were observed in U87 and U251 cells in comparison to NHAs (Figure 4D). The gene discussed is SFRP1; the disease is glioma.