On the other hand, lamin B1 reduction and nuclear softening occur in cell culture-based models of lung and breast cancer (Jia et al., 2019; Fischer et al., 2020), and knockout of lamin A/C or induction of heterochromatin decondensation are both sufficient to decrease nuclear stiffness (Lammerding et al., 2004; Lammerding et al., 2006; Nava et al., 2020). Here, LMNB1 is linked to breast cancer.