As we show in this study, chemogenetic inhibition of the release of NKB in the fPAG reversed the alleviating effect of Tacr3 overexpression on pT-ION-induced allodynia and anxiety-like behaviors, indicating that fPAGNKB → LHb regulates orofacial allodynia and anxiety-like behaviors in pT-ION mice. This evidence concerns the gene TACR3 and Anxiety.