In addition, although the central time and central distance in the OFT remained unchanged by CNO administration in the pT-ION+tacr3-over+hM4D(Gi)+CNO group (Figures 10Q, R; Figure S6B), the open-arm time, open-arm distance, and open-arm entries in the EPM were decreased (Figures 10O, P), indicating that pT-ION-induced anxiety-like behaviors reappeared after the release of NKB in the fPAG was inhibited. Here, TAC3 is linked to Anxiety.