CD8A and melanoma: Under in vitro co-culture conditions, B16F10 melanoma cells exhibited significant accumulation of 4-HNE when mixed with CD8+ T cells derived from lymph nodes draining histotripsy-treated B16F10 tumors; in contrast, CD8+ T cells derived from untreated B16F10 tumor-draining lymph nodes mediated no measurable ferroptosis (Figures 5I, J).