Immunohistochemical analysis of morphologically intact tumor cells at the junction of histotripsy ablated and non-ablated zones demonstrated rapid and transient colocalized upregulation of pRIPK3 and pMLKL (Figures 1F, G) indicating the onset of necroptosis, a TNFα-driven pathway of immunogenic cell death (46, 47). This evidence concerns the gene TNF and neoplasm.