TNF and neoplasm: Moreover, RNASeq of CD45- tumor cell populations performed 3 days after sham or histotripsy ablation revealed no clear differences in overall patterns of transcriptional activity between control tumors and histotripsy-abscopal tumors (Figure 2B); in contrast to control tumors, upregulated transcriptional activity of genes associated with necroptosis, ER stress, cellular response to LPS, TNFα signaling, and inflammatory responses were observed in histotripsy-treated tumors but not histotripsy-abscopal tumors (Figure 2C).