Finally, as in NASH diet–fed MCP-1ΔHep mice, hepatocyte MCP-1 ablation significantly ameliorated Notch-induced HSC activation (Figure 7, E and F), leading to lower hydroxyproline content (Figure 7G) and liver Sirius red staining (Figure 7H). This evidence concerns the gene CCL2 and metabolic dysfunction-associated steatohepatitis.