Next, to test the necessity of hepatocyte MCP-1 for NASH diet–induced inflammation and fibrosis, we generated hepatocyte-specific MCP-1–knockout (MCP-1ΔHep) mice by transducing MCP-1–floxed animals with AAV8-Tbg-Cre, which takes advantage of liver targeting of AAV8 and the hepatocyte-specific thyroxine binding globulin (Tbg) promoter (22) (Figure 3A). The gene discussed is SERPINA7; the disease is metabolic dysfunction-associated steatohepatitis.