The mRNA level of key glycolytic regulators like hexokinase 2 (HK2), 6‐phosphofructo‐ 2‐kinase /fructose‐2, 6‐bisphosphatase 3 (PFKFB3) and pyruvate kinase M2 (PKM2) were notably elevated in ASPP2‐depleted HCC‐LM3, HepG2 and Hep3B cells, whereas these were decreased in ASPP2‐overexpressed Huh‐7 cells (Figure 2A). This evidence concerns the gene PKM and hepatocellular carcinoma.