TARDBP and amyotrophic lateral sclerosis: On the contrary, when we used a primer pair that specifically recognizes the mutant sequence, we observed reduced levels of TDP-43 transcript in ALS fibroblasts treated with TDP-43i (ALS1A P = 0.0138; ALS2A P < 0.0001), but mostly, a significant reduction of TDP-43G376D mRNA levels after siM10 transfection (ALS1A P = 0.0018; ALS2A P = 0.0004; Fig. 3D).