To study the causal involvement of GPX8, we silenced its expression in Caki1 (using CRISPR-Cas9) and 786O (using dox-inducible shRNA) ccRCC cell lines that carry wild type (WT) and mutant (MT) VHL, respectively (Fig. 2A). Here, GPX8 is linked to nonpapillary renal cell carcinoma.