To this end, CRCs were induced in Trp53fl/fl or VP mice by AOM/DSS, followed by intraperitoneal injection with adeno-associated vectors (AAV) encoding WT-ATIC, ATIC-K266R, ATIC-K266Q or a control empty vector and ATIC expression was confirmed by immunohistochemistry (IHC) and western blot (Figs. 5a, 5b, S5a and S5b). Here, ATIC is linked to infectious otitis media.