We believe that the different responses of 22RV1 cells compared to co-culture of PC-3 and DU145 cells with osteoblasts may be related to the intrinsic properties of the three cell lines [3, 19], while overexpression of RBM3 inhibited the nuclear and total protein levels of β-catenin in PCa cells after co-culture. Here, RBM3 is linked to posterior cortical atrophy.