Given the role of macrophages in cancer therapy resistance (Klemm and Joyce, 2015; Ruffell and Coussens, 2015; Coffelt and de Visser, 2015) and their specific involvement in MycCaP-Bo bone metastasis from our analysis (Fig. 1), we stained macrophages using Iba1 and found enhanced infiltration of macrophages in MycCaP-Bo bone metastasis upon enzalutamide treatment (Fig. 2 G). This evidence concerns the gene AIF1 and cancer.