To understand the mechanism of how macrophages induced FN1-ITGA5 expression in bone-metastatic PC, RNA sequencing gene expression profiling was performed using FACS purified monocytes (CD45+CD11b+Ly-6ChiCCR2+) and macrophages (CD45+CD11b+Ly-6C−Ly-6G−F4/80+SSClow; gating strategy specified in Fig. S2 F; purity >96%) from naive tumors (vehicle) and resistant tumors (enzalutamide 18 d) as described in Fig. 4 A. This evidence concerns the gene ITGAM and pachyonychia congenita.