Then, the cellular potency and physicochemicalproperties were optimized through insights from molecular modelingstudies by implanting various solubilizing groups in phenyl ringsA and B. Optimized lead 52 shows 8-fold selective inhibitionof H1975 (EGFRL858R/T790M overexpressing) cancer cellsover A431 (EGFRWT overexpressing) cancer cells; westernblot analysis further confirmed EGFR mutant-selective target modulationinside the cancer cells by 52. This evidence concerns the gene EGFR and cancer.