SPECC1 and infection: In particular, we show that a lysine to aspartic acid change at position 294 (K294E) (i) reduces NSP2 CTR flexibility in silico, (ii) causes RV to form smaller, more numerous viroplasms during infection, (iii) decreases viroplasm fusion kinetics, (iv) reduces the number of VLS in NSP2/NSP5-coexpressing cells, and (v) mitigates the capacity of NSP2 and NSP5 to undergo LLPS in vitro.