TOP1 and Familial prostate cancer: We also compared TOP1cc signals with traditional ChIP–seq data targeting Top1 in human prostate cancer LNCAP cells (Extended Data Fig. 1a) and found that, although 62.3% TOP1cc proved to locate with Top1-enriched regions (Extended Data Fig. 1b–d), TOP1cc could be detected at only around 14.1% of the Top1-enriched regions detected with ChIP–seq29, which might reflect assay noise due to the formaldehyde fixation17.