Since no standard ofCS-CD44, ofCS-SDC1, and ofCS-CSPG4 are available, the sensitivity and specificity of the optimized ELISA were characterized by cancer cell line (SW480) spiked sample and CSA/decorin competitive assay including chondroitinase-treated cancer cell line or plasma samples (Fig. 2c, d). This evidence concerns the gene CSPG4 and cancer.