CASP3 and amyotrophic lateral sclerosis: As was found by immunofluorescence, there was no difference in cleaved caspase-3 expression levels in OrgCtrl cerebral organoids injected either with control or ALS patient-derived protein extracts, while in OrgALS cerebral organoids there was more cleaved caspase-3 expression with ALS patient-derived protein extracts (Fig 5H and 5I).