Although our cerebral organoids from iPSCs derived from an ALS-FTLD patient do not exhibit apparent cytoplasmic TDP-43 expression before the administration of ALS patient-derived protein extract, they may have a defect in nuclear-cytoplasmic TDP-43 translocations that facilitates TDP-43 aggregation once exposed to pathogenic TDP-43 from ALS. This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.