The statistical evaluation using densitometry in Western blot analysis also revealed that cleaved caspase-3 expression was the same in OrgCtrl cerebral organoids injected with control or ALS patient-derived protein extract, but it was significantly increased in OrgALS cerebral organoids injected with ALS patient-derived protein extracts (Fig 5J and 5K). The gene discussed is CASP3; the disease is amyotrophic lateral sclerosis.