To clarify the mechanism synergistic anti-cancer effect of FYLM, we investigated the protein expression of PRC1, Wnt pathway-related protein (β-catenin, c-Myc, c-Jun) and EGFR pathway-related protein (p-EGFR, EGFR, p-Akt and Akt) in xenograft tumor tissues by western blot and immunohistochemistry. Here, AKT1 is linked to cancer.