By venn diagram (Figure S2), 9 potential biomarkers including LPC 22:6, PC(16:0/20:4), PE(22:6/16:0), Cer(d18:1/24:0), Cer(d18:1/23:0), Cer(d18:1/22:0), TG(18:1/18:2/18:2), TG(16:0/16:0/20:3), and TG(18:0/16:0/18:2) (FDR < 0.05 and P < 0.05) were overlapping between 49 candidate lipids metabolites screened from non-targeted lipidomic data (discovery cohort) and 37 differential lipids from targeted lipidomic data (validation cohort), and they show similar significant trends in prediabetes and T2DM relative to controls (Table 2 and Figure 4). Here, CBLN1 is linked to type 2 diabetes mellitus.