Univariate analysis found that age >65, sex, NIHSS score, hypertension, history of the previous stroke, heart rate >100, aortic sinus diameter, left atrial diameter, CRP, platelet count, D-D dimer, triglyceride, high-density lipoprotein, TnT > 0.014, NT-proBNP > 270, HT, and multiple ischemic lesions in MRI were significantly different between AF and non-AF groups (P < 0.05 for all). The gene discussed is NPPB; the disease is atrial fibrillation.