ITGB3 and neoplasm: The Estimation of STromal and Immune cells in MAlignant Tumor tissues using Expression data (ESTIMATE) algorithm uses the gene enrichment analysis of a single sample and the gene expression profile of immune cells and stromal cells to infer the degree of infiltration of stromal and immune cells in tumor samples and estimate the purity of tumor cells based on the empirical cumulative distribution function (Supplementary Table 1).We investigated whether ITGβ3 expression was correlated with immune infiltration level in patients with COAD via the ESTIMATE algorithm.