Quantitative real-time reverse transcription-PCR (qRT-PCR) analyses further confirmed that upregulated MMP-14 transcript levels correlate with shorter metastasis-free survival in breast cancer (58) and that high MMP-11 mRNA levels independently predict the increased risk of distant metastasis in breast cancer, especially in HER2-positive breast cancer (53). This evidence concerns the gene MMP14 and breast carcinoma.