Weighted gene co-expression network analysis (WGCNA), protein–protein interaction (PPI) network mutual analysis, and the Kaplan–Meier survival prognosis analysis were used to identify six key genes associated with the prognosis of bladder cancer: VEGFA, ANXA1, HSP90B1, PSMA7, PRDX6, and PPP1CB. The gene discussed is PRDX6; the disease is urinary bladder cancer.