In conclusion, this study reveals the infiltrated cell composition in the tumor microenvironment of bladder cancer and expounds the high heterogeneity intra- and inter-tumor and the correlation between genes VEGFA, ANXA1, HSP90B1, PSMA7, PRDX6, and PPP1CB; the invasion degree of BC was elaborated, as well as the dynamic changes in the progression of BC, which confirms that they were highly correlated with the prognosis of the BC. Here, HSP90B1 is linked to urinary bladder cancer.