Taken together, we screened genes associated with radioresistance using sequencing data from HCT-116WT and HCT-116RR cells, and built a risk score model with five radioresistance genes, including TNFRSF13C, CD36, ANGPTL4, LAMB3, and SERPINA1. This risk score model showed favorable performance in prognosis prediction after radiotherapy for CRC. Here, SERPINA1 is linked to colorectal carcinoma.