To validate the activation of innate immune signaling in UNC93B1-high expressed AML, we next use GSEA analysis to investigate the enrichment pathway, and finally, we found four significantly activated pathways, which were innate immune system (NES = 2.67, p = 0.01, Figure 3C), immunoregulatory interactions between a lymphoid and a non-lymphoid cell (NES = 2.60, p = 0.01, Supplementary Figures S3A), neutrophil degranulation (NES = 2.23, p = 0.01, Supplementary Figures S3B) and adaptive immune system (NES = 2.94, p = 0.01, Supplementary Figure S3C; Supplementary Table S6). This evidence concerns the gene UNC93B1 and acute myeloid leukemia.