Finally, 14 TFs (NOTCH3, NR5A1, WDR5, RARB, SRCAP, MTA2, ONECUT1, PITX3, TRAF6, SMAD3, JDP2, FOSL1, GLI1, MTF1) were found to be significantly related to RFS in GC patients by multivariate Cox analysis. The gene discussed is SMAD3; the disease is gastric cancer.