Since activated microglia have been shown to secrete various pro-inflammatory cytokines such as IL-1β, IL-6, and TNF-α in various neurodegenerative diseases, we modeled the secretory effect of activated microglia by treating the control- and N4KO-Neurons with 50 nM IL-6 for 24 h, to recapitulate the humoral effect of activated microglia on the adjacent neurons. Here, TNF is linked to neurodegenerative disease.