Flow cytometry analysis of the B16F10 melanoma treated by two i.v. injections of free IL‐12 or Nano‐IL‐12 at 1 μg IL‐12 equivalence/injection showed that Nano‐IL‐12 significantly enhanced the infiltration of CD8+ T cells in the tumor compared to free IL‐12 (Figure 4a). The gene discussed is CD8A; the disease is neoplasm.