RUNX1 and acute myeloid leukemia: F2 double transgenic embryos were generated by crossing F1 Tg(Runx1:IDH2R172K) or Tg(Runx1:IDH2R140Q) to Tg(Runx1:FLT3ITD) [17] to investigate potential synergistic effects of these mutant genes which may co-exist in AML patients.