To further clarify whether the killing of malignant cells induced by APR-246 is dependent on mutant TP53, we used CRISPR/Cas9 technology to generate isogenic background human cancer cell lines of diverse origin that had different TP53 states owing to the removal of endogenous wt TP53 or mutant TP53, respectively (Supplementary Table S1). Here, TP53 is linked to cancer.