This included the breast cancer cell line MDA-MB-231 (R280K mutant TP53), the colorectal cancer cell lines HT29 (R273H mutant TP53) and SW620 (R273H and P309S mutant TP53), the Burkitt lymphoma cell line Rael-BL (R282W mutant TP53), the lung cancer cell line A549 (wt TP53) and the osteosarcoma cell line SJSA-1 (wt TP53) [24]. This evidence concerns the gene TP53 and osteosarcoma.