To investigate the function of PBX1 in colorectal tumors, we chose two cell lines, LoVo and HCT116, which had low PBX1expression levels in CRC cell lines (Supplementary Fig. S2A), to overexpress the PBX1 protein (Fig. 2A and Supplementary Fig. S2B). The gene discussed is PBX1; the disease is colorectal neoplasm.