Our in vitro assays show that CD24+CD44+CD54+EpCAM+ GCSCs exhibit the ability to differentiate and generate different subsets of cells with distinct phenotypes, such as the CD24−CD44−CD54-EpCAM− phenotype, similar to the results observed in the in vivo model, where the CD24+CD44+CD54+EpCAM+ GCSCs exhibited differentiation potential, generating specialized cells with the ability to secrete mucins (Fig. 5), a characteristic described for some GC types that indicates deeper invasion, greater size and higher metastatic potential. Here, CD44 is linked to gastric cancer.