In cancer development, the key regulators of the glycolytic enzyme consist of glucose transporter 1 (GLUT1), hexokinase1/2 (HK1/2), phosphofructokinase 2/fructose bisphosphatase 3 (PFK2/PFKFP3), phosphoglycerate kinase 1 (PGK1), pyruvate kinase 2 (PKM2), and lactate dehydrogenase (LDH). The gene discussed is PFKFB3; the disease is cancer.