TMEM105 and breast carcinoma: Additionally, we prepared three truncated mutants of TMEM105 promoter, with truncated mutations at position 1 (Promoter-MUT1-Luc), position 2 (Promoter-MUT2-Luc) or both positions (Promoter-MUT3-Luc), and cloned them into pGL3 vector and transfected them into breast cancer cells.